A simple, accurate and precise dual wavelength UV spectrophotometric method was developed for simultaneous determination of metformin hydrochloride and gliclazide in combined pharmaceutical dosage forms. The literature review reveals that there is no dual wavelength method was developed for this combination of drugs, hence this method was developed. The wavelengths selected for determination of metformin hydrochloride were 222.35 nm and 228.94 nm, whereas, the wavelengths selected for determination of gliclazide were 229.41 nm and 234.82 nm. Methanol and distilled water were used as the solvents. Regression analysis of Beer’s plots showed good correlation in concentration range of 5-30 μg/ml for metformin hydrochloride and 1-6 μg/ml for gliclazide. Accuracy of method was found between 98.0-102.0%. The precision (intra-day, inter-day and analyst to analyst) of method was found within limits (%CV<2). LOD was found to be 0.162 μg and 0.0825 μg for metformin hydrochloride and gliclazide respectively and LOQ was found to be 0.492 μg and 0.25 μg for metformin hydrochloride and gliclazide respectively. The proposed method was successfully applied to determination of these drugs in laboratory-prepared mixtures and commercial tablets.
Loading....